| dc.contributor.author | Keshavarz, M. | |
| dc.contributor.author | Skill, M. | |
| dc.contributor.author | Hollenhorst, M. I. | |
| dc.contributor.author | Maxeiner, S. | |
| dc.contributor.author | Walecki, M. | |
| dc.contributor.author | Pfeil, U. | |
| dc.contributor.author | Kummer, W. | |
| dc.contributor.author | Krasteva-Christ, G. | |
| dc.date.accessioned | 2022-11-18T09:53:06Z | |
| dc.date.available | 2019-05-15T12:43:45Z | |
| dc.date.available | 2022-11-18T09:53:06Z | |
| dc.date.issued | 2018 | |
| dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:hebis:26-opus-145667 | |
| dc.identifier.uri | https://jlupub.ub.uni-giessen.de//handle/jlupub/9407 | |
| dc.identifier.uri | http://dx.doi.org/10.22029/jlupub-8795 | |
| dc.description.abstract | The mechanisms of controlling airway smooth muscle (ASM) tone are of utmost clinical importance as inappropriate constriction is a hallmark in asthma and chronic obstructive pulmonary disease. Receptors for acetylcholine and serotonin, two relevant mediators in this context, appear to be incorporated in specialized, cholesterol-rich domains of the plasma membrane, termed caveolae due to their invaginated shape. The structural protein caveolin-1 partly accounts for anchoring of these receptors. We here determined the role of the other major caveolar protein, caveolin-3 (cav-3), in orchestrating cholinergic and serotonergic ASM responses, utilizing newly generated cav-3 deficient mice. Cav-3 deficiency fully abrogated serotonin-induced constriction of extrapulmonary airways in organ baths while leaving intrapulmonary airways unaffected, as assessed in precision cut lung slices. The selective expression of cav-3 in tracheal, but not intrapulmonary bronchial epithelial cells, revealed by immunohistochemistry, might explain the differential effects of cav-3 deficiency on serotonergic ASM constriction. The cholinergic response of extrapulmonary airways was not altered, whereas a considerable increase was observed in cav-3â -/- intrapulmonary bronchi. Thus, cav-3 differentially organizes serotonergic and cholinergic signaling in ASM through mechanisms that are specific for airways of certain caliber and anatomical position. This may allow for selective and site-specific intervention in hyperreactive states. | en |
| dc.language.iso | en | de_DE |
| dc.rights | Namensnennung 4.0 International | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject.ddc | ddc:610 | de_DE |
| dc.title | Caveolin-3 differentially orchestrates cholinergic and serotonergic constriction of murine airways | en |
| dc.type | article | de_DE |
| local.affiliation | FB 11 - Medizin | de_DE |
| local.opus.id | 14566 | |
| local.opus.institute | Institute of Anatomy and Cell Biology | de_DE |
| local.opus.fachgebiet | Medizin | de_DE |
| local.source.uri | https://doi.org/10.1038/s41598-018-25445-1 | |
| local.source.freetext | Scientific Reports 8(1):7508 | de_DE |
