Show simple item record

dc.contributor.authorLu, Yongning
dc.contributor.authorBhushan, Sudhanshu
dc.contributor.authorTchatalbachev, Svetlin
dc.contributor.authorMarconi, Marcelo
dc.contributor.authorBergmann, Martin
dc.contributor.authorWeidner, Wolfgang
dc.contributor.authorChakraborty, Trinad
dc.contributor.authorMeinhardt, Andreas
dc.date.accessioned2022-11-18T09:56:58Z
dc.date.available2013-03-01T13:21:42Z
dc.date.available2022-11-18T09:56:58Z
dc.date.issued2012
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:hebis:26-opus-92258
dc.identifier.urihttps://jlupub.ub.uni-giessen.de//handle/jlupub/9665
dc.identifier.urihttp://dx.doi.org/10.22029/jlupub-9053
dc.description.abstractMale infertility is a frequent medical condition, compromising approximately one in twenty men, with infections of the reproductive tract constituting a major etiological factor. Bacterial epididymo-orchitis results in acute inflammation most often caused by ascending canalicular infections from the urethra via the continuous male excurrent ductal system. Uropathogenic Escherichia coli (UPEC) represent a relevant pathogen in urogenital tract infections. To explore how bacteria can cause damage and cell loss and thus impair fertility, an in vivo epididymo-orchitis model was employed in rats by injecting UPEC strain CFT073 into the vas deference in close proximity to the epididymis. Seven days post infection bacteria were found predominantly in the testicular interstitial space. UPEC infection resulted in severe impairment of spermatogenesis by germ cell loss, damage of testicular somatic cells, a decrease in sperm numbers and a significant increase in TUNEL (+) cells. Activation of caspase-8 (extrinsic apoptotic pathway), caspase-3/−6 (intrinsic apoptotic pathway), caspase-1 (pyroptosis pathway) and the presence of 180 bp DNA fragments, all of which serve as indicators of the classical apoptotic pathway, were not observed in infected testis. Notably, electron microscopical examination revealed degenerative features of Sertoli cells (SC) in UPEC infected testis. Furthermore, the passive release of high mobility group protein B1 (HMGB1), as an indication of necrosis, was observed in vivo in infected testis. Thus, necrosis appears to be the dominant cell death pathway in UPEC infected testis. Substantial necrotic changes seen in Sertoli cells will contribute to impaired spermatogenesis by loss of function in supporting the dependent germ cells.en
dc.language.isoende_DE
dc.rightsNamensnennung 3.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/*
dc.subject.ddcddc:610de_DE
dc.titleNecrosis is the dominant cell death pathway in uropathogenic Escherichia coli elicited epididymo-orchitis and is responsible for damage of rat testisen
dc.typearticlede_DE
local.affiliationFB 11 - Medizinde_DE
local.opus.id9225
local.opus.fachgebietMedizinde_DE
local.source.urihttps://doi.org/10.1371/journal.pone.0052919
local.source.freetextPLoS ONE 8(1):e52919;de_DE


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record