Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling
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Heinze, Roger J.
Sekerina, Svetlana
Winkler, Ines
Biertuempfel, Christian
Oretskaya, Tatiana S.
Kubareva, Elena
Friedhoff, Peter
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http://dx.doi.org/10.22029/jlupub-15779Abstract
The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS-DNA complexes and will enable their structural and functional analysis.Link to publications or other datasets
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Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich. This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
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Molecular BioSystems 8(7):1861-1864 doi: 10.1039/c2mb25086a