Establishment of a perfusion cell culture system for bovine granulosa cells to investigate the relation between negative energy balance and declining fertility in dairy cattle
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Abstract
Fertility in dairy cattle is ubiquitously declining over the last decades. The situation is apparently linked to rising milk production and thus to the deficient metabolic situation in high yielding cows. Investigations on cellular level can elucidate fundamental underlying processes, in particular if the applied system allows simulating (patho-) physiological conditions. Therefore, a perfusion cell culture system was established for bovine granulosa cells (bGCs) and compared to a conventional, static culture system. Subsequently, the perfusion system was used to investigate the influence of negative energy balance-related metabolites on bGC function.Link to publications or other datasets
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Giessen : VVB Laufersweiler