Promicromonospora umidemergens sp. nov., isolated from moisture from indoor wall material

Abstract

A Gram-positive, yellow-pigmented, branched-hyphae-forming micro-organism, strain 09-Be-007T, was isolated from the wall of an indoor environment. Based on 16S rRNA gene sequence similarity studies, strain 09-Be-007T belonged to the genus Promicromonospora. The novel isolate showed sequence similarities of 98.8% to Promicromonospora aerolata V54AT, 98.9% to Promicromonospora vindobonensis V45T, 98.1% to Promicromonospora sukumoe DSM 44121T, 98.2% to Promicromonospora kroppenstedtii RS16T, 96.7% to Promicromonospora flava CC 0387T and 97.8% to Promicromonospora citrea DSM 43110T, the type strain of the type species of this genus. Cell wall sugars of strain 09-Be-007T were galactose, rhamnose and glucose. The diagnostic diamino acid of the cell-wall peptidoglycan was lysine. The major menaquinones detected were MK-9(H4) and MK-9(H6). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. Major fatty acids were iso-C15:0 and anteiso-C15:0; moderate amounts of anteiso-C17:0, iso-C16:0 and iso-C15:1 were also found. All these data supported the affiliation of strain 09-Be-007T to the genus Promicromonospora. DNA DNA hybridization values and physiological and biochemical data enabled strain 09-Be-007T to be differentiated genotypically and phenotypically from the six recognized species of the genus Promicromonospora. For these reasons, strain 09-Be-007T represents a novel species, for which the name Promicromonospora umidemergens sp. nov. is proposed, with 09-Be-007T (=DSM 22081T=CCM 7634T) as the type strain.